Liquid Teflon© and snug is all the head studs need to be (with very clean threads).
For the gaskets to seal properly you need a specific RA, I believe 60 RA or better is usually the requirement.
As for using O-rings, you will find that the manufacturers of the head gaskets will know more about use of them with their gaskets than anyone else. If I remember correctly, Cometic says to not use O-rings with their MLS head gaskets.
Usually a head gasket is used as a gauge. It is the one thing that is allowed to blow if undesirable conditions exist inside the cylinder. Rather than cracking pistons, bending rods, breaking pins or cranks, the head gaskets will blow, which is a much easier, cost effective part to replace. You then find out what caused the gasket to blow, such as too hot of combustion temperatures or too advanced timing, which will cause a massive spike in cylinder pressures, and a pressure wave that will break parts. What happens when racers break their forged crankshafts, they do not make them stronger and more bulky, they find out WHY they are breaking, and fix it.